Biostimulant Effects of Plant Proteins via Enzymatic Hydrolysis

Summary of Biostimulant action of a plant-derived protein hydrolysate produced through enzymatic hydrolysis

Biostimulant Effects of Plant Proteins on Crop Growth and Nitrogen Uptake

The Biostimulant Effects of Plant Proteins are increasingly recognized in sustainable agriculture for their ability to improve plant growth, nutrient uptake, and overall crop performance. This study investigated these effects using laboratory bioassays and greenhouse trials to evaluate hormone-like activity and crop productivity.

Laboratory Bioassays Confirm Biostimulant Activity

Two laboratory experiments highlighted the impact of plant proteins on growth. In corn (Zea mays L.), coleoptile elongation significantly increased with protein treatments, showing a dose-dependent response similar to indole-3-acetic acid. Tomato cuttings also exhibited improved rooting, with shoot and root dry weight, root length, and root area increasing by 21–35% at 6 ml/L. These results demonstrate the auxin-like activity behind the biostimulant effects of plant proteins.

Greenhouse Trials Demonstrate Enhanced Growth and Nitrogen Uptake

Greenhouse experiments confirmed positive growth responses in dwarf pea (Pisum sativum L.) and tomato (Solanum lycopersicum L.). Shoot length in dwarf pea increased by 33%, while tomato plants treated with protein solutions up to 10 ml/L showed increases of 20.5% in total dry biomass, 15% in SPAD index, and 21.5% in leaf nitrogen content. This evidence reinforces the beneficial role of plant proteins in promoting crop development.

Mechanisms Behind Biostimulant Activity

The growth-promoting effects of plant proteins are mainly attributed to amino acids and small peptides that trigger hormone-like responses and enhance nutrient absorption. Improved root architecture, shoot elongation, and nitrogen uptake contribute to stronger, more productive plants.

Conclusion: The study demonstrates that incorporating plant proteins as biostimulants can effectively improve plant growth, rooting, and nitrogen uptake, offering a sustainable approach to modern agriculture.